Lecture 7 - 9/12: Protein structure and function III Today's reading: Chapter 5 pp 166-179. Reading for next lecture: Make sure you've read Chapter 5 carefully. Key words: LIGAND and BINDING SITE ANTIBODIES PROTEIN PURIFICATION HOMOGENIZATION -> CELL EXTRACT CENTRIFUGATION GEL ELECTROPHORESIS COLUMN CHROMATOGRAPHY ION EXCHANGE, GEL-FILTRATION (size exclusion) and AFFINITY CHROMATOGRAPHY ISOELECTRIC FOCUSING and ISOELECTRIC POINT (pI) PEPTIDE SEQUENCING X-RAY CRYSTALOGRAPHY NMR